CDC25A (Phospho-Ser178) Antibody

Catalog Number:

11948

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P30304;
NCBI Gene#: 993;
NCBI Protein#: NP_001780.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 178(Q-N-S(p)-A-P) derived from Human CDC25A .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of CDC25A only when phosphorylated at serine 178.

Applications:

WB

Background:

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Chen MS, Ryan CE, Piwnica-Worms H (2003)Mol Cell Biol 23, 7488-97
Hassepass I, Voit R, Hoffmann I (2003) J Biol Chem 278, 29824-9
S?rensen CS, et al. (2003) Cancer Cell 3, 247-58

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF