MKP-1/2 (Phospho-Ser296/318) Antibody

Catalog Number:

11947

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P28562/Q13115;
NCBI Gene#: 1843/1846;
NCBI Protein#: NP_004408.1/NP_001385.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 296/318(I-I-S(p)-P-N) derived from Human MKP-1/2 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of MKP-1/2 only when phosphorylated at serine 296/318.

Applications:

WB

Background:

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

Lin YW, Yang JL (2006) J Biol Chem 281, 915-26

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF