Vimentin (Phospho-Ser56) Antibody

Catalog Number:

11940

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P08670;
NCBI Gene#: 7431;
NCBI Protein#: NP_003371.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 56 (A-S-S(p)-P-G) derived from Human Vimentin.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Vimentin only when phosphorylated at serine 56.

Applications:

WB

Background:

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

Matsuyama M, et al. (2013)J Biol Chem 288, 35626-35. Haolong C, et al. (2013)PLoS One 8, e73900. Kotula E, et al. (2013)PLoS One 8, e80313.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF