Product Datasheet  
Vimentin (Phospho-Ser56) Antibody  
Catalog Number: 11940  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species:  
  • Rabbit
  • Amount:  
  • 100μgμg
  • Swiss-Prot No.:  
  • Swiss-Prot#: P08670;
    NCBI Gene#: 7431;
    NCBI Protein#: NP_003371.2
  • Form of Antibody:  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage:  
  • Store at -20˚C/1 year
  • Immunogen:  
  • Peptide sequence around phosphorylation site of serine 56 (A-S-S(p)-P-G) derived from Human Vimentin.
  • reactivity:  
  • Hu
  • appl_detail:  
  • Western blotting: 1:500~1:1000
  • other_names:  
  • VIME; vimentin;
  • Purification:  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity:  
  • The antibody detects endogenous level of Vimentin only when phosphorylated at serine 56.
  • Applications:  
  • WB
  • Background:  
  • Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

    Matsuyama M, et al. (2013)J Biol Chem 288, 35626-35. Haolong C, et al. (2013)PLoS One 8, e73900. Kotula E, et al. (2013)PLoS One 8, e80313.




 
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