IKK- alpha/ beta (Phospho-Ser176/177) Antibody

Catalog Number:

11931

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O15111/O14920;
NCBI Gene#: 1147;
NCBI Protein#: NP_001269.3

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK-alpha/beta.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 176/177.

Applications:

WB

Background:

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3.

Chandrakesan P, et al. (2010)J Biol Chem 285, 33485-98
Hinz M, et al. (2010£© Mol Cell 40, 63-74
Choudhary S, Lu M, Cui R, Brasier AR (2007)Mol Endocrinol 21, 2203-17

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF