Product Datasheet  
IKK- alpha/ beta (Phospho-Ser176/177) Antibody  
Catalog Number: 11931  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: O15111/O14920;
    NCBI Gene#: 1147;
    NCBI Protein#: NP_001269.3
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK-alpha/beta.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Western blotting: 1:500~1:1000
  • other_names：  
  • FLJ40509; I-kappa-B kinase; IKBKB; kinase beta; NFKBIKB
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 176/177.
  • Applications：  
  • WB
  • Background：  

  • Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3.

    Chandrakesan P, et al. (2010)J Biol Chem 285, 33485-98
    Hinz M, et al. (2010£© Mol Cell 40, 63-74
    Choudhary S, Lu M, Cui R, Brasier AR (2007)Mol Endocrinol 21, 2203-17




 
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