IKK- alpha/ beta (Phospho-Ser180/181) Antibody

Catalog Number:

11930

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O15111/O14920;
NCBI Gene#: 1147;
NCBI Protein#: NP_001269.3

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 180/181 (C-T-S(p)-F-V) derived from Human IKK- alpha/ beta.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 180/181.

Applications:

WB

Background:

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.

Hafeez BB, et al. (2008) Cancer Res 68, 8564-72
Cahill CM, Rogers JT (2008) J Biol Chem 283, 25900-12
Encinas M, et al. (2008)Cell Death Differ 15, 1510-21

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF