Akt (Phospho-Ser129) Antibody

Catalog Number:

11914

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P31749;
NCBI Gene#: 207;
NCBI Protein#: NP_001014431.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine129(D-N-S(p)-G-A) derived from Human Akt .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Akt only when phosphorylated at serine 129.

Applications:

WB

Background:

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

Nguyen le XT, Mitchell BS (2013) Proc Natl Acad Sci U S A 110, 20681-6
Ponce DP, et al. (2011) Mol Cell Biochem 356, 127-32
Siddiqui-Jain A, et al. (2010) Cancer Res 70, 10288-98

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF