Product Datasheet  
Akt (Phospho-Ser129) Antibody  
Catalog Number: 11914  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P31749;
    NCBI Gene#: 207;
    NCBI Protein#: NP_001014431.1
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine129(D-N-S(p)-G-A) derived from Human Akt .
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Western blotting: 1:500~1:1000
  • other_names：  
  • AKT; AKT1 kinase; kinase Akt1; RAC; RAC-PK-alpha
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of Akt only when phosphorylated at serine 129.
  • Applications：  
  • WB
  • Background：  

  • General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

    Nguyen le XT, Mitchell BS (2013) Proc Natl Acad Sci U S A 110, 20681-6
    Ponce DP, et al. (2011) Mol Cell Biochem 356, 127-32
    Siddiqui-Jain A, et al. (2010) Cancer Res 70, 10288-98




 
© Signalway Biotechnology All Rights Reserved.