ATF1 (Phospho-Ser63) Antibody

Catalog Number:

11913

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P18846;
NCBI Gene#: 466;
NCBI Protein#: NP_005162.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 63(R-P-S(p)-Y-R) derived from Human ATF1 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of ATF1 only when phosphorylated at serine 63.

Applications:

WB

Background:

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.

Mackenzie KF, et al. (2013) J Immunol 190, 565-77
Dickinson RJ, et al. (2011) J Biol Chem 286, 38018-26
Wang J, Zhou JY, Wu GS (2011) J Biol Chem 286, 22384-92

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF