Smad2 (Phospho-Ser465) Antibody

Catalog Number:

11838

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q15796;
NCBI Gene#: 4087;
NCBI Protein#: NP_001003652.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 465(C-S-S(p)-M-S) derived from Human Smad2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Smad2 only when phosphorylated at serine 465.

Applications:

WB

Background:

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Riggins G.J., Nat. Genet. 13:347-349(1996).
Zhang Y., Nature 383:168-172(1996).
Eppert K., Cell 86:543-552(1996).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF