PLD1 (Phospho-Thr147) Antibody

Catalog Number:

11818

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q13393;
NCBI Gene#: 5337;
NCBI Protein#: NP_002653.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 147(R-H-T(p)-F-R) derived from Human PLD1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.

Applications:

WB

Background:

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis.

Hammond S.M., J. Biol. Chem. 272:3860-3868(1997) [PubMed: 9013646].
Steed P.M., FASEB J. 12:1309-1317(1998) [PubMed: 9761774].
Lopez I., J. Biol. Chem. 273:12846-12852(1998) [PubMed: 9582313].

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF