Product Datasheet  
PLD1 (Phospho-Thr147) Antibody  
Catalog Number: 11818  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q13393;
    NCBI Gene#: 5337;
    NCBI Protein#: NP_002653.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 147(R-H-T(p)-F-R) derived from Human PLD1.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000

  • other_names：  
  • PLD 1; hPLD1; Choline phosphatase 1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.
  • Applications：  
  • WB
  • Background：  

  • Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis.

    Hammond S.M., J. Biol. Chem. 272:3860-3868(1997) [PubMed: 9013646].
    Steed P.M., FASEB J. 12:1309-1317(1998) [PubMed: 9761774].
    Lopez I., J. Biol. Chem. 273:12846-12852(1998) [PubMed: 9582313].




 
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