PLD2 (Phospho-Tyr169) Antibody

Catalog Number:

11813

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O14939;
NCBI Gene#: 5338;
NCBI Protein#: NP_002654.3.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N) derived from Human PLD2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.

Applications:

WB IHC

Background:

Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.

Steed P.M., FASEB J. 12:1309-1317(1998).
Lopez I., J. Biol. Chem. 273:12846-12852(1998).
Divecha N., EMBO J. 19:5440-5449(2000).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF