Product Datasheet  
PLD2 (Phospho-Tyr169) Antibody  
Catalog Number: 11813  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: O14939;
    NCBI Gene#: 5338;
    NCBI Protein#: NP_002654.3.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N) derived from Human PLD2.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • PLD 2; PLD1C; choline phosphatase 2;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
  • Applications：  
  • WB IHC
  • Background：  

  • Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.

    Steed P.M., FASEB J. 12:1309-1317(1998).
    Lopez I., J. Biol. Chem. 273:12846-12852(1998).
    Divecha N., EMBO J. 19:5440-5449(2000).




 
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