p47 phox (Phospho-Ser345) Antibody

Catalog Number:

11811

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P14598;
NCBI Gene#: 653361;
NCBI Protein#: NP_000256.4.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 345(P-Q-S(p)-P-G) derived from Human Nuclear p47 phox.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of p47 phox only when phosphorylated at serine 345.

Applications:

WB IHC

Background:

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Lomax K.J., Science 245:409-412(1989).
Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF