Product Datasheet  
p47 phox (Phospho-Ser345) Antibody  
Catalog Number: 11811  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P14598;
    NCBI Gene#: 653361;
    NCBI Protein#: NP_000256.4.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 345(P-Q-S(p)-P-G) derived from Human Nuclear p47 phox.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • NCF1; P47 phox; NCF-47K;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of p47 phox only when phosphorylated at serine 345.
  • Applications：  
  • WB IHC
  • Background：  

  • NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

    Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
    Lomax K.J., Science 245:409-412(1989).
    Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).




 
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