CDC25C (Phospho-Ser198) Antibody

Catalog Number:

11790

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P30307;
NCBI Gene#: 995;
NCBI Protein#: NP_001274511.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 198(E-F-S(p)-L-K) derived from Human CDC25C.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of CDC25C only when phosphorylated at serine 198.

Applications:

IHC

Background:

cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Sadhu K., Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143(1990).
Bureik M., Int. J. Oncol. 17:1251-1258(2000).
Wegener S., Eur. J. Cell Biol. 79:810-815(2000).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF