B-Raf (Phospho-Thr753) Antibody

Catalog Number:

11786

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P15056;
NCBI Gene#: 673;
NCBI Protein#: NP_004324.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 753 (P-K-T(p)-P-L) derived from Human B-Raf.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 753

Applications:

WB

Background:

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992).
Albert S., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Scherer S.W., Science 300:767-772(2003)

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF