Product Datasheet  
B-Raf (Phospho-Thr753) Antibody  
Catalog Number: 11786  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P15056;
    NCBI Gene#: 673;
    NCBI Protein#: NP_004324.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 753 (P-K-T(p)-P-L) derived from Human B-Raf.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Western blotting: 1:500~1:1000

  • other_names：  
  • B-RAF; RAFB1; RMIL; p94;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 753
  • Applications：  
  • WB
  • Background：  

  • Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

    Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992).
    Albert S., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
    Scherer S.W., Science 300:767-772(2003)




 
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