ATF-2 (Phospho-Ser472) Antibody

Catalog Number:

11785

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P15336;
NCBI Gene#: 1386;
NCBI Protein#: NP_001243019.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 472 (A-L-S(p)-Q-I) derived from Human ATF-2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of ATF-2 only when phosphorylated at serine 472.

Applications:

IHC

Background:

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

Maekawa T., EMBO J. 8:2023-2028(1989).
Yang L., J. Immunol. 158:2522-2525(1997).
Kara C.J., Mol. Cell. Biol. 10:1347-1357(1990).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF