Product Datasheet  
ATF-2 (Phospho-Ser472) Antibody  
Catalog Number: 11785  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P15336;
    NCBI Gene#: 1386;
    NCBI Protein#: NP_001243019.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 472 (A-L-S(p)-Q-I) derived from Human ATF-2.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Immunohistochemistry: 1:50~1:100

  • other_names：  
  • Activating 2; ATF2; cAMP response element binding protein CRE- BP1; CREB2; HB16
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of ATF-2 only when phosphorylated at serine 472.
  • Applications：  
  • IHC
  • Background：  

  • Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

    Maekawa T., EMBO J. 8:2023-2028(1989).
    Yang L., J. Immunol. 158:2522-2525(1997).
    Kara C.J., Mol. Cell. Biol. 10:1347-1357(1990).




 
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