Connexin 43 (Phospho-Tyr265) Antibody

Catalog Number:

11767

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P17302;
NCBI Gene#: 2697;
NCBI Protein#: NP_000156.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Tyrosine 265(Q-K-Y(p)-A-Y) derived from Human Connexin 43.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

Connexin 43 antibody detects endogenous levels of Connexin 43 only when phosphorylated at Tyrosine 265

Applications:

WB

Background:

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Toshihiko Toyofuku, J. Biol. Chem., May 1998; 273: 12725.
Toshihiko Toyofuku, J. Biol. Chem., Jan 1998; 273: 1519.
Masayuki Shimada, Biol Reprod, Apr 2001; 64: 1255.
Merry L. Lindsey, Circulation, Jun 2006; 113: 2919 - 2928.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF