iNOS (Phospho-Tyr151) Antibody

Catalog Number:

11766

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P35228;
NCBI Gene#: 4843;
NCBI Protein#: NP_000616.3.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 151(Q-Y-Y(p)-G-S) derived from Human iNOS .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Inos only when phosphorylated at tyrosine 151.

Applications:

IHC

Background:

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Geller D.A., Proc. Natl. Acad. Sci. U.S.A. 90:3491-3495(1993).
Sherman P.A., Biochemistry 32:11600-11605(1993).
Charles I.G., Proc. Natl. Acad. Sci. U.S.A. 90:11419-11423(1993).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF