MYT1 (Phospho-Ser83) Antibody

Catalog Number:

11745

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q99640;
NCBI Gene#: 9088;
NCBI Protein#: XP_006721039.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 83(R-V-S(p)-F-R) derived from Human MYT1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of MYT1 only when phosphorylated at serine 83.

Applications:

IHC

Background:

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Booher R.N., J. Biol. Chem. 272:22300-22306(1997).
Liu F., Mol. Cell. Biol. 17:571-583(1997).
Nousiainen M., Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF