MAP3K1 (Phospho-Thr1400) Antibody

Catalog Number:

11737

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q13233;
NCBI Gene#: 4214;
NCBI Protein#: NP_005912.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 1400(K-G-T(p)-G-A) derived from Human MAP3K1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1400.

Applications:

WB IHC

Background:

Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.

Schmutz J., Nature 431:268-274(2004).
Xia Y., Genes Dev. 12:3369-3381(1998).
Vinik B.S., Mamm. Genome 6:782-783(1995)

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF