Product Datasheet  
MAP3K1 (Phospho-Thr1400) Antibody  
Catalog Number: 11737  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q13233;
    NCBI Gene#: 4214;
    NCBI Protein#: NP_005912.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 1400(K-G-T(p)-G-A) derived from Human MAP3K1.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • M3K1; MAP3K1; MAPKKK1; MEKK1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1400.
  • Applications：  
  • WB IHC
  • Background：  

  • Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.

    Schmutz J., Nature 431:268-274(2004).
    Xia Y., Genes Dev. 12:3369-3381(1998).
    Vinik B.S., Mamm. Genome 6:782-783(1995)




 
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