CK-1α (Phospho-Tyr294) Antibody

Catalog Number:

11728

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P48729/Q8N752;
NCBI Gene#: 1452/122011;
NCBI Protein#: NP_001883.4.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1α.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of CK-1α only when phosphorylated at tyrosine 294.

Applications:

WB IHC

Background:

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

Tapia C., FEBS Lett. 349:307-312(1994).
Fish K.J., J. Biol. Chem. 270:14875-14883(1995).
Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF