Product Datasheet  
CK-1α (Phospho-Tyr294) Antibody  
Catalog Number: 11728  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P48729/Q8N752;
    NCBI Gene#: 1452/122011;
    NCBI Protein#: NP_001883.4.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1α.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • KC1A; CKI-alpha; CSNK1A1; alpha isoform; Casein kinase I
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of CK-1α only when phosphorylated at tyrosine 294.
  • Applications：  
  • WB IHC
  • Background：  

  • Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

    Tapia C., FEBS Lett. 349:307-312(1994).
    Fish K.J., J. Biol. Chem. 270:14875-14883(1995).
    Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.




 
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