BLK (Phospho-Tyr501) Antibody

Catalog Number:

11727

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P51451;
NCBI Gene#: 640;
NCBI Protein#: NP_001706.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 501 (R-Q-Y(p)-E-L) derived from Human BLK.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of BLK only when phosphorylated at tyrosine 501.

Applications:

IHC

Background:

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor.

Islam K.B., J. Immunol. 154:1265-1272(1995).
Drebin J.A.,Oncogene 10:477-486(1995).
Greenman C.,Nature 446:153-158(2007).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF