Product Datasheet  
BLK (Phospho-Tyr501) Antibody  
Catalog Number: 11727  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P51451;
    NCBI Gene#: 640;
    NCBI Protein#: NP_001706.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 501 (R-Q-Y(p)-E-L) derived from Human BLK.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Immunohistochemistry: 1:50~1:100

  • other_names：  
  • p55-BLK; EC 2.7.10.2; kinase Blk; B lymphocyte kinase; Tyrosine-protein kinase BLK
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of BLK only when phosphorylated at tyrosine 501.
  • Applications：  
  • IHC
  • Background：  

  • Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor.

    Islam K.B., J. Immunol. 154:1265-1272(1995).
    Drebin J.A.,Oncogene 10:477-486(1995).
    Greenman C.,Nature 446:153-158(2007).




 
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