SHP-1 (Phospho-Tyr564) Antibody

Catalog Number:

11721

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P29350;
NCBI Gene#: 5777;
NCBI Protein#: NP_002822.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 564(D-V-Y(p)-E-N) derived from Human SHP-1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of SHP-1 only when phosphorylated at tyrosine 564.

Applications:

WB IHC IF

Background:

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Yi T., Mol. Cell. Biol. 12:836-846(1992).
Shen S.H., Nature 352:736-739(1991).
Plutzky J., Proc. Natl. Acad. Sci. U.S.A. 89:1123-1127(1992).

References:

appl_detail:

Western blotting: 1:500~1:1000
IHC dilution: 1:50-1:100
IF dilution: 1:100-1:200

Datasheet PDF