Retinoblastoma (Phospho-Ser608) Antibody

Catalog Number:

11720

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P06400;
NCBI Gene#: 5925;
NCBI Protein#: NP_000312.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 608(Y-L-S(p)-P-V) derived from Human Retinoblastoma.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Retinoblastoma only when phosphorylated at serine 608.

Applications:

WB IHC IF

Background:

Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases.

Lee W.-H., Nature 329:642-645(1987).
Lee W.-H., Science 235:1394-1399(1987).
Friend S.H., Proc. Natl. Acad. Sci. U.S.A. 84:9059-9063(1987).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
IF dilution: 1:100-1:200

Datasheet PDF