PDK1 (Phospho-Tyr9) Antibody

Catalog Number:

11719

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O15530;
NCBI Gene#: 5170;
NCBI Protein#: NP_002604.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 9(A-L-Y(p)-D-A) derived from Human PDK1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of PDK1 only when phosphorylated at tyrosine 9.

Applications:

IHC

Background:

Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.

Alessi D.R., Curr. Biol. 7:261-269(1997).
Alessi D.R.,Curr. Biol. 7:776-789(1997).
Stephens L.R.,Science 279:710-714(1998)

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF