eNOS (Phospho-Thr495) Antibody

Catalog Number:

11711

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P29474;
NCBI Gene#: 4846;
NCBI Protein#: NP_000594.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 495 (K-K-T(p)-F-K derived from Human eNOS .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of eNOS only when phosphorylated at threonine 495.

Applications:

WB

Background:

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Janssens S.P., J. Biol. Chem. 267:14519-14522(1992).
Janssens S.P., J. Biol. Chem. 267:22694-22694(1992).
Marsden P.A., FEBS Lett. 307:287-293(1992)

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF