Product Datasheet  
eNOS (Phospho-Thr495) Antibody  
Catalog Number: 11711  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P29474;
    NCBI Gene#: 4846;
    NCBI Protein#: NP_000594.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 495 (K-K-T(p)-F-K derived from Human eNOS .
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000

  • other_names：  
  • cNOS; EC-NOS; ECNOS; NOS; NOS3
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of eNOS only when phosphorylated at threonine 495.
  • Applications：  
  • WB
  • Background：  

  • Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

    Janssens S.P., J. Biol. Chem. 267:14519-14522(1992).
    Janssens S.P., J. Biol. Chem. 267:22694-22694(1992).
    Marsden P.A., FEBS Lett. 307:287-293(1992)




 
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