c-Jun (Phospho-Ser63) Antibody

Catalog Number:

11710

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P05412;
NCBI Gene#: 3725;
NCBI Protein#: NP_002219.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 63(L-T-S(p)-P-D) derived from Human c-Jun.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The Antibody detects endogenous levels of c-Jun only when phosphorylated at Serine 63.

Applications:

WB IHC

Background:

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Wei W, et al. (2005). Cancer Cell. 8(1): 25-33.
Sevilla A, et al. (2004). Oncogene.23(55): 8950-8958.
Li L, et al. (2004). J Biol Chem.279(6): 4058-4065.
Hurd C, et al. (2002). Oncogene.21(14): 2154-2160.

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF