CD227/MUC1 (Phospho-Tyr1229) Antibody

Catalog Number:

11707

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P15941;
NCBI Gene#: 4582;
NCBI Protein#: NP_001018016.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y(p)-E-K) derived from Human CD227/MUC1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.

Applications:

WB IHC IF

Background:

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

Lan M.S., J. Biol. Chem. 265:15294-15299(1990).
Ligtenberg M.J.L., J. Biol. Chem. 265:5573-5578(1990).
Gendler S.J., J. Biol. Chem. 265:15286-15293(1990)

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF