Product Datasheet  
CD227/MUC1 (Phospho-Tyr1229) Antibody  
Catalog Number: 11707  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P15941;
    NCBI Gene#: 4582;
    NCBI Protein#: NP_001018016.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y(p)-E-K) derived from Human CD227/MUC1.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200

  • other_names：  
  • PUM; EMA; H23AG; MUC-1; PEM
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.
  • Applications：  
  • WB IHC IF
  • Background：  

  • The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

    Lan M.S., J. Biol. Chem. 265:15294-15299(1990).
    Ligtenberg M.J.L., J. Biol. Chem. 265:5573-5578(1990).
    Gendler S.J., J. Biol. Chem. 265:15286-15293(1990)




 
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