Nuclear Receptor NR4A1 (Phospho-Ser351) Antibody

Catalog Number:

11704

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P22736;
NCBI Gene#: 3164;
NCBI Protein#: NP_002126.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine351(L-P-S(p)-K-P) derived from Human Nuclear Receptor NR4A1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Nuclear Receptor NR4A1 only when phosphorylated at serine 351

Applications:

IHC

Background:

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.

Nakai A., Mol. Endocrinol. 4:1438-1443(1990).
Chang C., J. Steroid Biochem. 34:391-395(1989).
Bondy G.P., Cell Growth Differ. 2:203-208(1991)

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF