PKA-R2β (Phospho-Ser114) Antibody

Catalog Number:

11692

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P31323;
NCBI Gene#: 5577;
NCBI Protein#: NP_002727.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine114(R-A-S(p)-V-C) derived from Human PKA-R2β.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of PKA-R2β only when phosphorylated at serine 114.

Applications:

WB

Background:

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Levy F.O., Mol. Endocrinol. 2:1364-1373(1988).
DeGiorgis J.A., J. Proteome Res. 4:306-315(2005).
Han G., Proteomics 8:1346-1361(2008)

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF