B-RAF (Phospho-Thr599) Antibody

Catalog Number:

11682

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P15056;
NCBI Gene#: 673;
NCBI Protein#: NP_004324.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 599 (L-A-T(p)-V-K) derived from Human B-RAF.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.

Applications:

WB IHC IF

Background:

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418.
Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 - 35949.
R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 - 6953.
Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.

References:

appl_detail:

WB dilution: 1:500-1:1000
Immunohistochemistry: 1:50~1:100
IF dilution: 1:100-1:200

Datasheet PDF