Product Datasheet  
B-RAF (Phospho-Thr599) Antibody  
Catalog Number: 11682  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P15056;
    NCBI Gene#: 673;
    NCBI Protein#: NP_004324.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 599 (L-A-T(p)-V-K) derived from Human B-RAF.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • WB dilution: 1:500-1:1000
    Immunohistochemistry: 1:50~1:100
    IF dilution: 1:100-1:200
  • other_names：  
  • B-RAF; RAFB1; RMIL; BRAF1; c-RMIL
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.
  • Applications：  
  • WB IHC IF
  • Background：  

  • Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

    Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418.
    Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 - 35949.
    R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 - 6953.
    Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.




 
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