Bloom Syndrome (Phospho-Thr99) Antibody

Catalog Number:

11681

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P54132;
NCBI Gene#: 641;
NCBI Protein#: NP_000048.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.

Applications:

WB IHC

Background:

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).

Ellis N.A., Cell 83:655-666(1995).
Karow J.K., J. Biol. Chem. 272:30611-30614(1997).
Pichierri P., EMBO J. 23:3154-3163(2004).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF