Product Datasheet  
Bloom Syndrome (Phospho-Thr99) Antibody  
Catalog Number: 11681  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P54132;
    NCBI Gene#: 641;
    NCBI Protein#: NP_000048.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.
  • Applications：  
  • WB IHC
  • Background：  

  • Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).

    Ellis N.A., Cell 83:655-666(1995).
    Karow J.K., J. Biol. Chem. 272:30611-30614(1997).
    Pichierri P., EMBO J. 23:3154-3163(2004).




 
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