Bax (Phospho-Thr167) Antibody

Catalog Number:

11680

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q07812;
NCBI Gene#: 581;
NCBI Protein#: NP_620116.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine167 (P-G-T(p)-P-T) derived from Human Bax.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Bax only when phosphorylated at threonine 167.

Applications:

IF

Background:

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Francesca Andriani. J Natl Cancer Inst, Sep 2001; 93: 1314 - 1324.
H Martinez-Valdez. J. Exp. Med., Mar 1996; 183: 971.
Joslyn K. Brunelle. J. Biol. Chem., Feb 2004; 279: 4305 - 4312.

References:

appl_detail:

Immunofluorescence: 1:100~1:200

Datasheet PDF