SF1 (Phospho-Ser82) Antibody

Catalog Number:

11669

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q15637;
NCBI Gene#: 7536;
NCBI Protein#: NP_004621.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 82(S-P-S(p)-P-E) derived from Human SF1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of SF1 only when phosphorylated at serine 82.

Applications:

WB IHC

Background:

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

Susan Y. Park, Development, May 2005; 132: 2415 - 2423.
Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986.
Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839.
Philipp Gut,Development, Oct 2005; 132: 4611 - 4619.

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF