Product Datasheet  
SF1 (Phospho-Ser82) Antibody  
Catalog Number: 11669  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q15637;
    NCBI Gene#: 7536;
    NCBI Protein#: NP_004621.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 82(S-P-S(p)-P-E) derived from Human SF1.
  • reactivity：  
  • Hu Ms
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • BBP; CW17; SF01; ZFM1; ZNF162
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of SF1 only when phosphorylated at serine 82.
  • Applications：  
  • WB IHC
  • Background：  

  • Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

    Susan Y. Park, Development, May 2005; 132: 2415 - 2423.
    Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986.
    Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839.
    Philipp Gut,Development, Oct 2005; 132: 4611 - 4619.




 
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