p90 RSK (Phospho-Thr573) Antibody

Catalog Number:

11665

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q15418;
NCBI Gene#: 6195;
NCBI Protein#: NP_002944.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 573(L-M-T(p)-P-C) derived from Human p90 RSK.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of P90RSK only when phosphorylated at threonine 573.

Applications:

WB IHC

Background:

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes.

Eiichi Takahashi, J. Biol. Chem., Jul 1999; 274: 20206.
Ye Zhang, J. Biol. Chem., May 2002; 277: 19042 - 19048.
Ganesh R. Panta, Mol. Cell. Biol., Mar 2004; 24: 1823 - 1835.

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF