Product Datasheet  
p90 RSK (Phospho-Thr573) Antibody  
Catalog Number: 11665  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q15418;
    NCBI Gene#: 6195;
    NCBI Protein#: NP_002944.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 573(L-M-T(p)-P-C) derived from Human p90 RSK.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • KS6A1; p90RSK1; RSK1; RPS6KA1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of P90RSK only when phosphorylated at threonine 573.
  • Applications：  
  • WB IHC
  • Background：  

  • Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes.

    Eiichi Takahashi, J. Biol. Chem., Jul 1999; 274: 20206.
    Ye Zhang, J. Biol. Chem., May 2002; 277: 19042 - 19048.
    Ganesh R. Panta, Mol. Cell. Biol., Mar 2004; 24: 1823 - 1835.




 
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